In this post, we will learn about the different types of common laboratory animals which we often use in our Pharmacology Lab.
Guinea pigs (400-600g) are the commonly used experimental animals. they are very docile and easy to raise and maintain they are highly sensitive to histamine. They are used in experimental asthma to study bronchodilators. they are also used to local anaesthetics and as a model in amoebiasis and cholera as they are sensitive to this microorganism.
The white rat (200-250g) is the commonest laboratory animal used in experimental pharmacology. Rats are easy to breed and maintain. Resemble man in several organ function and nutrition and sensitive to most of the drugs; make them very useful experimental animals. However, they do not have a vomiting centre. The various rat tissue used is colon, stomach, uterus, caecum and vas deference. Besides these organs rat brain tissue is extensively employed in radio receptor-ligand studies. The other strains of rats are Sprague- Dawley and porton.
White mice are the smallest laboratory animals used. Mice are also easy to breed and maintain. They are small in size (25-30g) and therefore, easy to breed and maintain. They are sensitive to most of the drugs used in experimental pharmacology. Mice are used extensively in toxicity study, bioassay of insulin, testing of analgesics, CNS active drugs and chemotherapeutic agents. More recently mouse brains, as well as primary cell culture ofmouse spinal cord neurons, are used in neuropharmacology for studying neurotransmitters receptor functions the other strains of mice used are laca and balb/C.
Domestic rabbits (2-3 kg) are generally used for pyrogen testing. Some of the tissues or organs from rabbits used are the heart, aorta, duodenum and ileum. One peculiar thing about rabbits is that they are resistant to the actions of atropine as they contain the atropine-esterase enzyme, the presence of which is genetically determined.
Frogs (150-200g) were one time extensively used in experimental pharmacology. However, recently the use of wild frogs for experimental purposes has been banned. Earlier, frogs were used for isolated heart, rectus abdominis muscle preparation, the study of muscle nerve and ciliary movements, respectively. Frogs were also used for the study of nerve block type of local anaesthetics. frogs are inexpensive and easily available, and the ban on the use of frogs has been debated.
Cats, dogs and monkeys are used for pharmacological investigations of drugs. Cats and dogs were one time commonly used to study blood pressure experiments. But their use has been now restricted. However, beagle dogs are the only strain approved by regulatory authorities (USFDA) for preclinical testing of new drugs.
Alternatives to animal experimentation
Because of the growing concern about the use of animals in biomedical research in several countries have passed legislation to prevent or usage of animal experimentation. these include experiments with tissue and body fluids of normal animals and human use of microorganisms, primary cell culture and cell lines, use of models and computer simulation and software are being used nowadays. As per the common laboratory alternate animals.
|Common Name||Scientific Name||Used In|
|Zebra fish||Danio rerio||Developmental assay, Drug discovery, Neurobiology, Toxicity|
|Worms||Habolitis||Developmental assay, Neurobiology, Toxicity|
|Fruit fly||Drosophila||Cancer biology, Developmental assay, Neuroscience|
|Tunicates||Ciona sp||Developmental assay, Neuroscience|
|Starfish||Echinoderms||Developmental assay, neuroscience|