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Identification of Bacteria using Staining techniques
Staining is a technique used in microscopy to enhance contrast in microscopic images. Stains and dyes are frequently used in biological tissues for viewing, often with the aid of different microscopes.
IMViC reactions are a set of four useful reactions that are commonly employed in the identification of members of the family Enterobacteriaceae. The four reactions are the Indole test, Methyl Red test, Voges Proskauer test, and Citrate utilization test. The letter “i” is only for rhyming purposes.
Sterilization is a process that eliminates (removes) or kills all forms of life, including transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.) present on a surface, contained in a fluid, in medication, or in a compound such as biological culture media
Isolation and preservation methods for pure cultures
A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into a new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample many times before inoculating the new medium.
Bacterial Growth Curve
Most bacteria divide in a short amount of time and produce a large number of bacteria – easier to represent these large numbers by logarithmic scales
Cultivation of Anaerobs
Anaerobes are the predominant components of the skin’s and mucous membranes normal flora – cause infections of endogenous origin
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