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Aim: To determine the haemoglobin content in 20µl of the blood sample.
Principle: A hemoprotein composed of globin and heme that gives red blood cells their characteristic colour; functions primarily to transport oxygen from the lungs to the body tissues. The red blood cells are broken down with hydrochloric acid to get the haemoglobin into a solution. The free haemoglobin is exposed for a while to form hemin crystals. The solution is diluted to compare with a standard colour.
Requirements: Hemometer, Single mark pipette, Distilled water, Needle, Spirit, Cotton, HCl.
- Take 1/10 HCl in the Hb tube upto the lowest mark ‘2’.
- Prick the finger with needle and collect 20µl of blood sample with single mark pipette
- Place the Hb tube on the working table for five minutes for the formation of hemin crystals.
- Place the Hb tube in the compater/hemometer and add drop by drop of distilled water into it until the colour of the solution in the Hb tube coincides with the glass plates of the compater.
- If the colour coincides with the glass plates of the compater, observe the reading in the Hb tube. The percentage of Hb can be calculated from the reading.
Evaluation: Hb content in grams X 100 / 14.5
- Normal Values:
- Male: 14 to 18grams
- Females: 13 to 14grams
- Children: 10 to 13grams
Result: The hemoglobin content present in 20µl of blood sample is _____________________.