HPTLC PDF Note Free Download For Pharmacy students.
The Principle of separation is adsorption.
One or more compounds are spotted on a thin layer of adsorbent coated on a chromatographic plate.
The mobile phase (developing solvent) flows through because of capillary action (against gravitational force).
The compounds move according to their affinities towards the adsorbent.
The component with more affinity towards the stationary phase travels slower. The component with lesser affinity
towards the stationary phase travels faster.