Chemical Tests for Honey

Aim

To identify the chemical characters of the given sample.

Chemical Tests and Observations

TestObservationInference
Fiehe’s test:
Take about 3ml of honey + 2ml of
ether and shake thoroughly and allow
the 2 layers to separate and evaporate
to dryness. The upper etherial layer is
separated and put in a china dish and
evaporate, to the residue add 1%
resorcinol and HCl.
Transient pink colour


Permanent red colour
Pure honey


Adulterated
honey(Invert sugar)
Molisch’s Test:
Honey is treated with alpha Naphthol and concentrated sulphuric acid
Purple colourPresence of
carbohydrate
Reducing Sugar Test:
Heat honey to this add a drop of
a mixture of Fehling’s solution A & B
The brick red colour of
cuprous oxide
Presence of
monosaccharide

Conclusion

From the above morphological characters and chemical tests, the given crude drug is identified as Honey.

References

1.Kokate CK. Practical Pharmacognosy, 4 edition, VallabhPrakashan. Delhi; 1994: 98

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