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Pharmaceutical Biotechnology Notes free download for GPAT, NIPER, and University Semester Exam.
Pharmaceutical Biotechnology Notes 6th Semester PDF Free Download – 2021
Introduction to Biotechnology
- Brief introduction to Biotechnology with reference to Pharmaceutical Sciences.
- Enzyme Biotechnology- Methods of enzyme immobilization and applications.
- Biosensors- Working and applications of biosensors in Pharmaceutical Industries.
- Brief introduction to Protein Engineering.
- Use of microbes in industry. Production of Enzymes- General consideration – Amylase, Catalase, Peroxidase, Lipase, Protease, Penicillinase.
- Basic principles of genetic engineering
- Study of cloning vectors, restriction endonucleases and DNA ligase.
- Recombinant DNA technology. Application of genetic engineering in medicine.
- Application of r DNA technology and genetic engineering in the products:Interferon b) Vaccines- hepatitis- B c) Hormones- Insulin
- Brief introduction to PCR
- Types of immunity-humoral immunity, cellular immunity.
Types of Immunity, Humoral Immunity and Cellular Immunity
- Structure of Immunoglobulins, Structure and Function of MHC
- Hypersensitivity reactions, Immune stimulation and Immune suppressions.
- General method of the preparation of bacterial vaccines, toxoids, viral vaccine, antitoxins, serum-immune blood derivatives and other products relative to immunity.
- Storage conditions and stability of official vaccines
- Hybridoma technology- Production, Purification and Applications
- Immuno blotting techniques- ELISA, Western blotting, Southern blotting.
- Genetic organization of Eukaryotes and Prokaryotes
- Microbial genetics including transformation, transduction, conjugation, plasmids and transposons.
- Introduction to Microbial biotransformation and applications.
- Blood Products: Collection, Processing and Storage of whole human blood, dried human plasma, plasma Substituties.
- Fermentation methods and general requirements, study of media, equipments, sterilization methods, aeration process, stirring.
- Large scale production fermenter design and its various controls.
- Study of the production of – penicillins, citric acid, Vitamin B12, Glutamic acid, Griseofulvin
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